ABSTRACT
<p><b>OBJECTIVE</b>To develop a RP-HPLC method for determination of three glycosides in Swertia punicea.</p><p><b>METHOD</b>Chromatographic column: Alltimal C18 (4.6 mm x 250 mm, 5 microm). Mobile phase: methanol-water (including 0.05% H3PO4), and gradient elution. Flow rate: 1 mL x min(-1). Wavelength: 254 nm. Column temperture: 30 degrees C.</p><p><b>RESULT</b>The calibration curves of gentiopicroside, mangiferin and swertrianolin were in good linearity over the range of 31.3-281.7, 0.31-2.78, 0.55-4.91 microg, (r = 0.9996, 0.9993, 0.9995). The average recoveries were 103.36%, 101.42% and 97.39%, with RSD less then 3% (n = 5).</p><p><b>CONCLUSION</b>It is a simple and sensitive meathod in controlling the quality of S. punicea.</p>
Subject(s)
Calibration , Chromatography, High Pressure Liquid , Methods , Drugs, Chinese Herbal , Chemistry , Reference Standards , Glucosides , Glycosides , Iridoid Glucosides , Iridoids , Plants, Medicinal , Chemistry , Quality Control , Reproducibility of Results , Swertia , Chemistry , XanthonesABSTRACT
<p><b>OBJECTIVE</b>To explore the activities of Composite Artemisia Capillaris Tablet (CACT) against hepatitis B virus replication in vitro.</p><p><b>METHODS</b>By means of radioimmunoassay (RIA), Dot blot and Southern blot, the surface and e antigen production of 2.2.15 cells, HBV DNA in 2.2.15 cell culture medium and that in 2.2.15 cells were examined respectively.</p><p><b>RESULTS</b>HBsAg, HBeAg values of 2.2.15 cells treated by CACT were lower than those of the control, the HBV DNA quantities in culture medium and in 2.2.15 cells decreased as compared with those cells with no treatment by CACT given to them.</p><p><b>CONCLUSION</b>CACT could inhibit HBV DNA replication, showing its potential antiviral activity in hepatitis B treatment.</p>